The team also placed forward Kevin Porter and defenseman David Worsofsky on waivers with the purpose of assignment.
41-year-old defenseman Sergei Gonchar has been released from his professional tryout.
Pouliot was Pittsburgh’s first round pick, eighth overall, in the 2012 draft.
The 21-year-old scored two goals and seven points while averaging 17:32 of ice time in 34 NHL games last season.
The Penguins have to be disappointed that Pouliot couldn’t solidify a spot on the roster after his extended audition in 2014-15.
The news was much better for rookie forward Daniel Sprong, who remains on the roster.
At the very least, it looks like the 18-year-old will start the season with the Pens.
The 2015 second round pick can still be sent back to junior at any time.
Pittsburgh has 23 players remaining on their roster, but that doesn’t include injured forwards Eric Fehr and Tom Kuhnhackl.